How our technology visualizes DNA

Our test systems, which we use in our service laboratory and were developed in-house, are based on DNAstrip technology. We use this to detect bacterial or human DNA specifically.

PCR tubes

Every living organism possesses its own genetic information with an individual sequence in the form of DNA. This results in a uniquely personal genetic fingerprint that can be visualized using molecular techniques. Medical diagnostic testing makes use of this. Based on the detected DNA sequences, specific statements can be made, for example, about which bacterial species are causing the infection or whether the patient has a particular gene variant that is typical of a disease.

Hain Lifescience dental diagnostics

In the dental practice, molecular diagnostic testing provides crucial information for successful periodontal therapy. We offer our test systems micro-IDent® and micro-IDent®plus for this. These are based on DNA•strip technology, which we developed ourselves to the highest quality standards. It combines high sensitivity with maximum specificity and offers a high level of diagnostic reliability.

DNA•strip technology in detail

As soon as we receive the specimens from the dental practice, the cells they contain are broken open using special extraction methods and the DNA is isolated. The nucleic acids are then amplified selectively by means of the polymerase chain reaction (PCR). In the next step, the double-stranded amplificates created by this process are chemically denatured, because detection on the DNAstrip is carried out using single-stranded DNA.

The DNAstrip is a membrane strip made from a special carrier material. The DNAstrip is coated with highly specific probes which are complementary to the selectively amplified nucleic acid. The single-stranded amplificate binds specifically to the sensors during hybridization, while nonspecifically bound amplificates are removed in subsequent washing steps.

During the conjugate reaction, the specifically bound amplificate is marked with the enzyme alkaline phosphatase and is then made visible in an enzymatic color reaction. In this step, a specific banding pattern develops on the DNAstrip.

To evaluate the DNAstrip, we use a computer-based system which enables us to guarantee objective test results. After verification by our technical personnel, the data determined in the laboratory are forwarded to our customer service department. Here, the result reports are prepared and sent out. These show you the patient’s individual test results at a glance.