DNA•strip technology in detail
As soon as we receive the specimens from the dental practice, the cells they contain are broken open using special extraction methods and the DNA is isolated. The nucleic acids are then amplified selectively by means of the polymerase chain reaction (PCR). In the next step, the double-stranded amplificates created by this process are chemically denatured, because detection on the DNA•strip is carried out using single-stranded DNA.
The DNA•strip is a membrane strip made from a special carrier material. The DNA•strip is coated with highly specific probes which are complementary to the selectively amplified nucleic acid. The single-stranded amplificate binds specifically to the sensors during hybridization, while nonspecifically bound amplificates are removed in subsequent washing steps.
During the conjugate reaction, the specifically bound amplificate is marked with the enzyme alkaline phosphatase and is then made visible in an enzymatic color reaction. In this step, a specific banding pattern develops on the DNA•strip.
To evaluate the DNA•strip, we use a computer-based system which enables us to guarantee objective test results. After verification by our technical personnel, the data determined in the laboratory are forwarded to our customer service department. Here, the result reports are prepared and sent out. These show you the patient’s individual test results at a glance.